Alkaline Phosphatase anticorps

N° du produit ABIN7649384

L’anticorps Souris Monoclonal anti-Alkaline Phosphatase a été validé pour . Il convient pour détecter Alkaline Phosphatase dans des échantillons de Humain.

Aperçu rapide pour Alkaline Phosphatase anticorps (ABIN7649384)

Antigène: Alkaline Phosphatase (ALP)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Alkaline Phosphatase est non-conjugé

Application: Veuillez nous consulter SVP

Clone: ALPP-516

Détail du produit anti-Alkaline Phosphatase anticorps

Fonction: Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)(ALPP/516)

Attributs du produit: This antibody reacts with placental alkaline phosphatase. There are at least four distinct but related alkaline phosphatases: intestinal, placental (PLAP), placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, zinc metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This MAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human ALPP protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: HepG2 cells. Placenta or seminoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet:

Synonyms: Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1

Gene Symbol: ALPP

Poids moléculaire: 70 kDa

ID gène: 250

UniProt: P05187