Calcineurin B anticorps

N° du produit ABIN7650393

Cet anticorps anti-Calcineurin B est un anticorps Souris Monoclonal détectant Calcineurin B dans . Adapté pour Humain et Xenopus laevis.

Aperçu rapide pour Calcineurin B anticorps (ABIN7650393)

Antigène: Calcineurin B (CAN)

Reactivité: Humain, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calcineurin B est non-conjugé

Application: Veuillez nous consulter SVP

Clone: BE2-1

Détail du produit anti-Calcineurin B anticorps

Fonction: Calcineurin B / PPP3R1(BE2.1)

Attributs du produit: This antibody recognizes the Calcineurin B alpha protein, encoded by the PPP3R1 (Protein Phosphatase 3 Regulatory Subunit B Alpha) gene. Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human Calcineurin B protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Calcineurin B

Antigène: Calcineurin B (CAN)

Autre désignation: Calcineurin B

Sujet:

Synonyms: Calcineurin B, Calcineurin subunit B type 1, Protein Phosphatase 3 Regulatory Subunit B Alpha, CALNB1, Cna2, CNB, CNB1, PPP3R1

Gene Symbol: PPP3R1

Tissue Expression: Brain|T-cells

Poids moléculaire: 19 kDa

ID gène: 5534

UniProt: P63098

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling