Recombinant Caldesmon anticorps (High Molecular Weight) (Biotin)

N° du produit ABIN7650416

L’anticorps Souris Monoclonal anti-Caldesmon a été validé pour WB, IHC et IHC (fp). Il convient pour détecter Caldesmon dans des échantillons de Humain.

Aperçu rapide pour Recombinant Caldesmon anticorps (High Molecular Weight) (Biotin) (ABIN7650416)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCALD1-820

Détail du produit anti-Caldesmon anticorps (Biotin)

Épitope: High Molecular Weight

Fonction: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), Biotin conjugate

Attributs du produit: Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human CALD1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: Caldesmon

Sujet:

Synonyms: Caldesmon, Caldesmon 1, Caldesmon 1 Isoform 1, CDM, HCAD

Gene Symbol: CALD1

Tissue Expression: Smooth muscle

Poids moléculaire: 150 kDa

ID gène: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction