CA9 anticorps (CF®740)

N° du produit ABIN7650487

Cet anticorps Souris Monoclonal détecte spécifiquement CA9 dans WB, IHC et IHC (fp). Il présente une réactivité envers Humain et Cheval.

Aperçu rapide pour CA9 anticorps (CF®740) (ABIN7650487)

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Reactivité: Humain, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CA9 est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CA9-781

Détail du produit anti-CA9 anticorps (CF®740)

Fonction: Carbonic Anhydrase IX(CA9/781), CF740 conjugate

Attributs du produit: Carbonic anhydrase IX (carbonic anhydrase 9) is one of several carbolic anhydrases that vary in tissue distribution and localization. Carbonic anhydrases catalyze the interconversion of carbon dioxide and water into carbonic acid and bicarbonate and are found in all mammalian tissues. Carbonic anhydrase IX is a transmembrane protein that is not expressed in most healthy tissues, but is expressed in most renal cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CA9 protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CA9

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: Carbonic Anhydrase IX

Sujet:

Synonyms: Carbonic anhydrase IX, Carbonic anhydrase 9, RCC-associated antigen G250, Carbonic dehydratase, CA-IX, Membrane antigen MN

Gene Symbol: CA9

Tissue Expression: Kidney

Poids moléculaire: 55 kDa

ID gène: 768

UniProt: Q16790