Recombinant beta Catenin anticorps

N° du produit ABIN7650641

L’anticorps anti-beta Catenin Monoclonal Lapin est utilisé pour la détection de beta Catenin dans des échantillons de Humain. Il a été validé pour WB, IHC, FACS, IF et IHC (fp).

Aperçu rapide pour Recombinant beta Catenin anticorps (ABIN7650641)

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Catenin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CTNNB1-2030R

Détail du produit anti-beta Catenin anticorps

Fonction: Catenin, beta (CTNNB1) (CTNNB1/2030R)

Attributs du produit: Beta-catenin associates with the cytoplasmic portion of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion, including adherens junctions. Beta-catenin is involved in the Wnt signaling pathway as well as other signaling pathways, and is also found in complexes with many different proteins including the tumor suppressor protein APC. Defects in beta-catenin are associated with colorectal cancer, as well as many other cancer types. Beta-catenin is normally localized to the cell membrane, but can translocate to the nucleus in response to certain cell signaling. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human i²-catenin protein

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur beta Catenin

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Autre désignation: Beta-catenin

Sujet:

Synonyms: Beta-catenin, Catenin beta-1, Catenin (Cadherin associated protein), beta 1, CTNNB

Gene Symbol: CTNNB1

Tissue Expression: Epithelial cells

Poids moléculaire: 92 kDa

ID gène: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism