CDX2 anticorps

N° du produit ABIN7650758

L’anticorps Souris Monoclonal anti-CDX2 a été validé pour IHC et IHC (fp). Il convient pour détecter CDX2 dans des échantillons de Humain.

Aperçu rapide pour CDX2 anticorps (ABIN7650758)

Antigène: CDX2 (Caudal Type Homeobox 2 (CDX2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDX2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PCRP-CDX2-1A3

Détail du produit anti-CDX2 anticorps

Fonction: CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) (PCRP-CDX2-1A3)

Attributs du produit: The specificity of this monoclonal antibody to its intended target was validated by HuProt™Array, containing more than 19,000, full-length human proteins.The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human CDX2protein

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Immunoprecipitation 2-4 μg/mg protein. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HT29 cells or Colon Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CDX2

Antigène: CDX2 (Caudal Type Homeobox 2 (CDX2))

Autre désignation: CDX2

Sujet:

Synonyms: Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2,CDX2

Gene Symbol: CDX2

Tissue Expression: Intestine

Poids moléculaire: 40 kDa

ID gène: 1045

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance