CD16 anticorps (CF®740)

N° du produit ABIN7650820

Cet anticorps Souris Monoclonal détecte spécifiquement CD16 dans FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour CD16 anticorps (CF®740) (ABIN7650820)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est conjugé à/à la CF®740

Application: Flow Cytometry (FACS)

Clone: HuNK2

Détail du produit anti-CD16 anticorps (CF®740)

Fonction: CD16(HuNK2), CF740 conjugate

Attributs du produit: Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV, Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This MAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human peripheral blood lymphocytes from a NK-leukemia patient.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 ug/mL. Functional Studies order Ab without BSA & azide). Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet:

Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, SELPLG

Gene Symbol: FCGR3A

Tissue Expression: Monocytes/macrophages|Neutrophils|NK cells

Poids moléculaire: 50-80 kDa

ID gène: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process