CD16 anticorps (CF®740)

N° du produit ABIN7650821

L’anticorps Souris Monoclonal anti-CD16 a été validé pour FACS et IF. Il convient pour détecter CD16 dans des échantillons de Humain.

Aperçu rapide pour CD16 anticorps (CF®740) (ABIN7650821)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C16-1045

Détail du produit anti-CD16 anticorps (CF®740)

Fonction: CD16(C16/1045), CF740 conjugate

Attributs du produit: This antibody recognizes CD16 (Fci³RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (Fci³RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fci³RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CD16 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet:

Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, SELPLG

Gene Symbol: FCGR3A

Tissue Expression: Monocytes/macrophages|Neutrophils|NK cells

Poids moléculaire: 50-80 kDa

ID gène: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process