Recombinant CD34 anticorps (CF®740)

N° du produit ABIN7651289

L’anticorps anti-CD34 Monoclonal Lapin est utilisé pour la détection de CD34 dans des échantillons de Humain et Rat. Il a été validé pour FACS, IHC et IHC (fp).

Aperçu rapide pour Recombinant CD34 anticorps (CF®740) (ABIN7651289)

Antigène: CD34

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: HPCA1-1806R

Détail du produit anti-CD34 anticorps (CF®740)

Fonction: CD34 (Hematopoietic Stem Cell & Endothelial Marker) (HPCA1/1806R), CF740 conjugate

Attributs du produit: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human HPCA1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (formalin) 2-4 μg/mL. Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: KG-1 cells, Tonsil, or Angiosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet:

Synonyms: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Gene Symbol: CD34

Tissue Expression: Endothelial cells|Hematopoietic stem cells

Poids moléculaire: 90-110 kDa

ID gène: 947

UniProt: P28906