CD40 Ligand anticorps (AA 108-261) (CF®405S)

N° du produit ABIN7651440

Cet anticorps anti-CD40 Ligand est un anticorps Souris Monoclonal détectant CD40 Ligand dans FACS, IHC, IF et IHC (fp). Adapté pour Humain.

Aperçu rapide pour CD40 Ligand anticorps (AA 108-261) (CF®405S) (ABIN7651440)

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 Ligand est conjugé à/à la CF®405S

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CD40LG-2761

Détail du produit anti-CD40 Ligand anticorps (CF®405S)

Épitope: AA 108-261

Fonction: CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)(CD40LG/2761), CF405S conjugate

Attributs du produit: CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa 108-261) of human CD40LG protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: Jurkat cells. Tonsil, Spleen or Thymus.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD154

Sujet:

Synonyms: CD154, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein, TrAP, Tumor necrosis factor ligand superfamily member 5 (TNFSF5)

Gene Symbol: CD40LG

Poids moléculaire: 36 kDa

ID gène: 959

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints