Cyclin B1 anticorps (CF®740)

N° du produit ABIN7652735

L’anticorps Souris Monoclonal anti-Cyclin B1 a été validé pour IF et FACS. Il convient pour détecter Cyclin B1 dans des échantillons de Humain, Souris et Hamster.

Aperçu rapide pour Cyclin B1 anticorps (CF®740) (ABIN7652735)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Souris, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est conjugé à/à la CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: V92-1

Détail du produit anti-Cyclin B1 anticorps (CF®740)

Fonction: Cyclin B1(V92.1), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Hamster Cyclin B1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1

Sujet:

Synonyms: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Gene Symbol: CCNB1

Poids moléculaire: 55-62 kDa

ID gène: 891

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase