Recombinant EGFRviii anticorps (CF®647)

N° du produit ABIN7653357

Cet anticorps anti-EGFRviii Monoclonal Lapin conjugué à CF®647 (Clone GFR-2600R) (ABIN7653357) détecte spécifiquement EGFRviii dans IHC et IHC (fp). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant EGFRviii anticorps (CF®647) (ABIN7653357)

Antigène: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFRviii est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GFR-2600R

Détail du produit anti-EGFRviii anticorps (CF®647)

Fonction: EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), CF647 conjugate

Attributs du produit: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: A431 cells. Placenta, Breast, Colon or Bladder Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur EGFRviii

Antigène: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Autre désignation: EGFRvIII

Sujet:

Synonyms: Erbb1 variant III, ERBB1 variant III, HER1 variant III, Proto-oncogene c-ErbB-1 variant III, Receptor Tyrosine Protein Kinase variant III, ErbB1 variant III

Gene Symbol: EGFR

Poids moléculaire: ~145 kDa (vIII variant)

ID gène: 1956

UniProt: P00533