Recombinant Elastase 3B anticorps (AA 82-238) (CF®647)

N° du produit ABIN7653384

Cet anticorps anti-Elastase 3B est un anticorps Lapin Monoclonal détectant Elastase 3B dans IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour Recombinant Elastase 3B anticorps (AA 82-238) (CF®647) (ABIN7653384)

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Elastase 3B est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CELA3B-2809R

Détail du produit anti-Elastase 3B anticorps (CF®647)

Épitope: AA 82-238

Fonction: CELA3B / ELA3B (Pancreatic Function Marker) (CELA3B/2809R), CF647 conjugate

Attributs du produit: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CELA3B protein fragment (around aa 82-238)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Panc-28 or PANC1 cells. Pancreas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Elastase 3B

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Autre désignation: CELA3B

Sujet:

Synonyms: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E

Gene Symbol: CELA3B

Poids moléculaire: 17 kDa

ID gène: 23436

UniProt: P08861