EPO anticorps (AA 28-162) (CF®740)

N° du produit ABIN7653729

Cet anticorps anti-EPO est un anticorps Souris Monoclonal détectant EPO dans WB, IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour EPO anticorps (AA 28-162) (CF®740) (ABIN7653729)

Antigène: EPO (Erythropoietin (EPO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPO est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: EPO-1368

Détail du produit anti-EPO anticorps (CF®740)

Épitope: AA 28-162

Fonction: Erythropoietin (EPO) (EPO/1368), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of human EPO protein (aa28-162)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin) 4-8 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HepG2 Cells. Heart or Kidney.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur EPO

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin

Classe de substances: Hormone

Sujet:

Synonyms: EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2

Gene Symbol: EPO

Poids moléculaire: 37 kDa

ID gène: 2056

UniProt: P01588

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity