ERCC1 anticorps (AA 191-281) (CF®488A)

N° du produit ABIN7653881

L’anticorps Souris Monoclonal anti-ERCC1 a été validé pour . Il convient pour détecter ERCC1 dans des échantillons de Humain.

Aperçu rapide pour ERCC1 anticorps (AA 191-281) (CF®488A) (ABIN7653881)

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERCC1 est conjugé à/à la CF®488A

Application: Veuillez nous consulter SVP

Clone: ERCC1-2683

Détail du produit anti-ERCC1 anticorps (CF®488A)

Épitope: AA 191-281

Fonction: ERCC1 / RAD10 (Tumor Progression Marker)(ERCC1/2683), CF488A conjugate

Attributs du produit: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa 191-281) of human ERCC1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: HeLa, HepG2 cells. Tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur ERCC1

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Autre désignation: ERCC1

Sujet:

Synonyms: COFS4, DNA excision repair protein ERCC1, ERCC1, Excision repair cross complementing 1, RAD10, UV20

Gene Symbol: ERCC1

Poids moléculaire: ~110 kDa

ID gène: 2067

UniProt: P07992

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response