Fc gamma RII (CD32) anticorps (CF®740)

N° du produit ABIN7653982

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, WB, IP et Func. Il présente une réactivité envers Humain.

Aperçu rapide pour Fc gamma RII (CD32) anticorps (CF®740) (ABIN7653982)

Antigène: Fc gamma RII (CD32)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®740

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 7-3

Détail du produit

Fonction: CD32(7.3), CF740 conjugate

Attributs du produit: This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: K562 and FcgRII L cells

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K-562 cells. Lymph node, tonsil and placenta.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet:

Synonyms: CD32A, Fc fragment of IgG low affinity IIa receptor, Fc gamma RIIa, Fc-gamma RII-a, Fc-gamma-RIIa, FCGR2A, FcgRII, Fcr-2, FcRII-a, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17

Gene Symbol: FCGR2A

Tissue Expression: Endothelial cells

Poids moléculaire: 40 kDa

ID gène: 2212

UniProt: P12318