Integrin beta 3 anticorps (CF®740)

N° du produit ABIN7655447

L’anticorps Souris Monoclonal anti-Integrin beta 3 a été validé pour IHC et IHC (fp). Il convient pour détecter Integrin beta 3 dans des échantillons de Humain.

Aperçu rapide pour Integrin beta 3 anticorps (CF®740) (ABIN7655447)

Antigène: Integrin beta 3 (ITGB3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 3 est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: Y2-51

Détail du produit anti-Integrin beta 3 anticorps (CF®740)

Fonction: CD61 / Integrin beta-3 / Platelet Glycoprotein IIIa (Platelet Marker) (Y2/51), CF740 conjugate

Attributs du produit: This antibody reacts with human integrin beta-3 (GPIIIa, vitronectin receptor beta chain). It associates with the alphaalpha-V-chain (CD51) to form vitronectin receptor, or with the alphaalpha-IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Phytohemagglutinin (PHA)-stimulated peripheral blood cells

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (frozen or formalin) 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: U937, KG1a, HEL cells. Spleen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Integrin beta 3

Antigène: Integrin beta 3 (ITGB3)

Autre désignation: CD61

Sujet:

Synonyms: BDPLT2, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta-3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet glycoprotein IIIa, PTP

Gene Symbol: ITGB3

Tissue Expression: Platelets|Megakaryocytes

Poids moléculaire: 105 kDa & 90 kDa

ID gène: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex