LIG1 anticorps

N° du produit ABIN7656378

Cet anticorps anti-LIG1 est un anticorps Souris Monoclonal détectant LIG1 dans IP, FACS et Func. Adapté pour Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour LIG1 anticorps (ABIN7656378)

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Functional Studies (Func)

Clone: 1A9

Détail du produit anti-LIG1 anticorps

Fonction: DNA Ligase 1(1A9)

Attributs du produit: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Full-length native cow LIG1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Western blot 0.5-1 μg/mL, ELISA, Immunoprecipitation, Optimal dilution for a specific application should be determined.

Commentaires:

Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: DNA Ligase 1

Sujet:

Synonyms: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1

Gene Symbol: LIG1

Poids moléculaire: 133 kDa

ID gène: 3978

UniProt: P18858

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA