HLA-B anticorps (CF®740)

N° du produit ABIN7656463

Cet anticorps Souris Monoclonal détecte spécifiquement HLA-B dans FACS et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour HLA-B anticorps (CF®740) (ABIN7656463)

Antigène: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-B est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: JOAN-1

Détail du produit anti-HLA-B anticorps (CF®740)

Fonction: HLA-B(JOAN-1), CF740 conjugate

Attributs du produit: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Normal human peripheral blood lymphocytes

Isotype: IgG3, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Human peripheral blood lymphocytes, tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur HLA-B

Antigène: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Autre désignation: HLA-B

Sujet:

Synonyms: HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B

Gene Symbol: HLA-B

Poids moléculaire: 45 kDa

ID gène: 3106

UniProt: P03989

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response