MMP2 anticorps (AA 444-575) (CF®740)

N° du produit ABIN7656706

Cet anticorps Souris Monoclonal détecte spécifiquement MMP2 dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour MMP2 anticorps (AA 444-575) (CF®740) (ABIN7656706)

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MMP2 est conjugé à/à la CF®740

Application: Western Blotting (WB)

Clone: MMP2-1501

Détail du produit anti-MMP2 anticorps (CF®740)

Épitope: AA 444-575

Fonction: MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) (MMP2/1501), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human MMP2 protein fragment (aa444-575)

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: U-138 MG or U-87 MG cells. Placenta

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MMP2

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: Collagenase-Type-IV-A

Sujet:

Synonyms: Type IV collagenase|CLG4A|Collagenase Type 4 alpha|Collagenase type IV A|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metalloproteinase-2|MMP2|MONA|Neutrophil gelatinase|PEX|TBE-1

Gene Symbol: MMP2

Poids moléculaire: 72 kDa (Pro), 63 kDa (cleaved)

ID gène: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response