MUC5AC anticorps (CF®740)

N° du produit ABIN7657442

L’anticorps Souris Monoclonal anti-MUC5AC a été validé pour WB et RIA. Il convient pour détecter MUC5AC dans des échantillons de Humain, Souris, Singe, Cat et Boeuf (Vache).

Aperçu rapide pour MUC5AC anticorps (CF®740) (ABIN7657442)

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivité: Humain, Souris, Singe, Cat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC5AC est conjugé à/à la CF®740

Application: Western Blotting (WB), Radioimmunoassay (RIA)

Clone: 2-11M1

Détail du produit anti-MUC5AC anticorps (CF®740)

Fonction: MUC5AC (Mucin 5AC / Gastric Mucin)(2-11M1), CF740 conjugate

Attributs du produit: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively, it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Immunogène: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotype: IgG1, kappa

Information d'application

Indications d'application: ELISA: Use at 2-4 μg/mL for coating. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7 cells. Human colon or stomach (IHC).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MUC5AC

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Autre désignation: MUC5AC

Sujet:

Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Gene Symbol: MUC5AC

Tissue Expression: Epithelial cells

Poids moléculaire: >1,000 kDa

ID gène: 4586

UniProt: P98088