MSH6 anticorps (AA 374-540) (CF®640R)

N° du produit ABIN7657521

Cet anticorps anti-MSH6 est un anticorps Souris Monoclonal détectant MSH6 dans WB, IHC, IF et IHC (fp). Adapté pour Humain.

Aperçu rapide pour MSH6 anticorps (AA 374-540) (CF®640R) (ABIN7657521)

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est conjugé à/à la CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MSH6-3085

Détail du produit anti-MSH6 anticorps (CF®640R)

Épitope: AA 374-540

Fonction: MSH6 (DNA Mismatch Repair Protein)(MSH6/3085), CF640R conjugate

Attributs du produit: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of human MSH6 protein (around aa 374-540)

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: HCT116, MCF-7, A431 or HeLa cells. Colon Carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6

Sujet:

Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein

Gene Symbol: MSH6

Poids moléculaire: 85 kDa

ID gène: 2956

UniProt: P52701

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response