Recombinant Neurofilament anticorps

N° du produit ABIN7657894

Cet anticorps Lapin Monoclonal détecte spécifiquement Neurofilament dans IHC et IHC (fp). Il présente une réactivité avec des échantillons de Humain, Rat, Poulet, Porc et Boeuf (Vache).

Aperçu rapide pour Recombinant Neurofilament anticorps (ABIN7657894)

Antigène: Neurofilament

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Poulet, Porc, Boeuf (Vache)

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NEFL-2983R

Détail du produit anti-Neurofilament anticorps

Fonction: Neurofilament (NF-L) (Neuronal Marker) (NEFL/2983R)

Attributs du produit: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human NEFL protein

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Brain, Neuroblastoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Neurofilament

Antigène: Neurofilament

Sujet:

Synonyms: 68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68

Gene Symbol: NEFL

Tissue Expression: Neuroendocrine cells|Neurons

Poids moléculaire: 68 kDa

ID gène: 4747

UniProt: P07196