Nucleolin anticorps (CF®740)

N° du produit ABIN7658112

Cet anticorps anti-Nucleolin est un anticorps Souris Monoclonal détectant Nucleolin dans WB, IHC, IF, FACS et IHC (fp). Adapté pour Humain.

Aperçu rapide pour Nucleolin anticorps (CF®740) (ABIN7658112)

Antigène: Nucleolin (NCL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 364-5

Détail du produit anti-Nucleolin anticorps (CF®740)

Fonction: Nucleolin(364-5), CF740 conjugate

Attributs du produit: Recognizes Nucleolin (NCL), the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11 kb. It has a predicted molecular weight of ~76 kDa with an apparent MW of 100-110 kDa, attributed to phosphorylation of its N-terminal domain. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde- or methanol-fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Lysate of SU-DHL-1 Nuclei

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with mouse, rat or cow, others not known. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: All human cells

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin

Sujet:

Synonyms: NCL, Nucl, Nucleolin, Protein C23

Gene Symbol: NCL

Poids moléculaire: 76 kDa (predicted), 100-110 kDa (apparent)

ID gène: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization