GFP Tag anticorps

N° du produit ABIN7673315

Cet anticorps Lapin Polyclonal détecte spécifiquement GFP Tag dans WB, ELISA et IP. Il présente une réactivité envers Aequorea victoria.

Aperçu rapide pour GFP Tag anticorps (ABIN7673315)

Antigène: GFP Tag

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-GFP Tag anticorps

Fonction: GFP tag antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: full length

Immunogen: GFP tag

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000-1:4000, IP: 1:2000-1:20000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur GFP Tag

Antigène: GFP Tag

Classe de substances: Tag

Sujet:

Synonyms: CFP|eGFP|eYFP|GFP|GFP tag|YFP

Background: Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9 kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

Poids moléculaire: 30 kDa