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GFP Tag anticorps (AA 1-238) (HRP)

L’anticorps Souris Monoclonal anti-GFP Tag a été validé pour WB. Il convient pour détecter GFP Tag dans des échantillons de Aequorea victoria.
N° du produit ABIN7673385

Aperçu rapide pour GFP Tag anticorps (AA 1-238) (HRP) (ABIN7673385)

Antigène

GFP Tag

Reactivité

  • 23
  • 2
  • 1
  • 1
Aequorea victoria

Hôte

  • 18
  • 12
Souris

Clonalité

  • 19
  • 11
Monoclonal

Conjugué

  • 17
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp GFP Tag est conjugé à/à la HRP

Application

  • 22
  • 14
  • 9
  • 8
  • 6
  • 5
  • 3
Western Blotting (WB)

Clone

0D8
  • Épitope

    • 1
    • 1
    AA 1-238

    Fonction

    HRP-GFP tag Monoclonal antibody

    Purification

    Protein A+G purification

    Pureté

    ≥95 % as determined by SDS-PAGE

    Immunogène

    Immunogen sequence: 1-238aa

    Immunogen: GFP tag

    Isotype

    IgG1
  • Indications d'application

    WB: 1:2000-1:10000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freeze / thaw cycles.

    Stock

    -20 °C

    Stockage commentaire

    -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

    Date de péremption

    12 months
  • Antigène

    GFP Tag

    Classe de substances

    Tag

    Sujet

    Synonyms: CFP|eGFP|eYFP|GFP|GFP tag|YFP

    Background: Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9 kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

    Poids moléculaire

    30 kDa

    UniProt

    P42212
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