Recombinant CD32/CD16 anticorps

N° du produit ABIN7795363

Cet anticorps anti-CD32/CD16 Monoclonal Human (Clone 2-4G2) (ABIN7795363) détecte spécifiquement CD32/CD16 dans FACS, IHC, WB, ELISA, ICC et in vivo. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour Recombinant CD32/CD16 anticorps (ABIN7795363)

Antigène: CD32/CD16

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp CD32/CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)

Classe de qualité: In vivo Grade

Clone: 2-4G2

Détail du produit anti-CD32/CD16 anticorps

Expression System: Mammalian cells

Fonction: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: 2.4G2)

Attributs du produit: The recombinant 2.4G2 antibody binds to mouse CD16 (FcγIII) and CD32 (FcγII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (FcγI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications. The most popular mouse Fc blocking reagents include: Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining, it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity. Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls. Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking. Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. Best choice of mouse Fc blocking reagents based on your application are: For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2). For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. For Western Blot and ELISA: BSA or FBS. For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

Purification: Protein A or G affinity column

Pureté: >95 % by reducing SDS-PAGE

niveau d'endotoxine: < 1 EU per 1 mg of the protein by the LAL method

Isotype: Fc Silenced, IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: > 3 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Détails sur CD32/CD16

Antigène: CD32/CD16

Autre désignation: CD16/CD32