MPL anticorps (AA 26-595)

N° du produit ABIN7825562

Cet anticorps anti-MPL est un anticorps Lapin Polyclonal détectant MPL dans WB, FACS et ELISA. Adapté pour Humain.

Aperçu rapide pour MPL anticorps (AA 26-595) (ABIN7825562)

Antigène: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MPL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Détail du produit anti-MPL anticorps

Épitope: AA 26-595

Fonction: Anti-TPOR/MPL Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-TPOR/MPL Antibody catalog # A00483-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human TPOR/MPL recombinant protein (Position: Q26-Q595). Human TPOR/MPL shares 79.6% amino acid (aa) sequence identity with mouse TPOR/MPL.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur MPL

Antigène: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Autre désignation: MPL

Sujet: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Poids moléculaire: 90 kDa

ID gène: 352

UniProt: P40238

Pathways: Signalistation JAK/STAT