Recombinant Cyclin B1 anticorps

N° du produit ABIN7828138

Cet anticorps Lapin Monoclonal détecte spécifiquement Cyclin B1 dans WB, IF et IP. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Cyclin B1 anticorps (ABIN7828138)

Antigène: Cyclin B1 (CCNB1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: A546

Détail du produit anti-Cyclin B1 anticorps

Fonction: Recombinant Cyclin B1 Monoclonal Antibody

Purification: Protein A purified

Immunogène: Recombinant human Cyclin B1 fragment

Isotype: IgG, kappa

Information d'application

Indications d'application: WB 1:500-1:1000,IF 1:50,IP 1:50-1:100

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Date de péremption: 12 months

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1

Sujet: G2 mitotic specific cyclin B,G2/mitotic-specific cyclin-B,Cyclin B,CCNB,CCNB1,Cyclin B1,CCNB 1,G2 mitotic specific cyclin B1,G2/mitotic-specific cyclin-B1,Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Poids moléculaire:

Calculated MW: 55 kDa

Observed MW: 55 kDa

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase