eGFP anticorps

N° du produit ABIN7833234

Cet anticorps Lapin Polyclonal détecte spécifiquement eGFP dans WB et ICC. Il présente une réactivité avec des échantillons de Aequorea victoria.

Aperçu rapide pour eGFP anticorps (ABIN7833234)

Antigène: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp eGFP est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Détail du produit anti-eGFP anticorps

Fonction: Anti-EGFP | Enhanced Green Fluorescence Protein

Réactivité croisée (Details): GFP, EGFP
Not reactive in: mCherry

Purification: Immunogen affinity purified

Immunogène: Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli

Information d'application

Indications d'application: 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water, ultrapure water

Buffer: Immunogen affinity purified in PBS pH 7.2-7.6, 0.1 % trehalose, 5 mM sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store lypholized antibody at 4 °C. After reconstitution keep aliquots at -20°C for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened

Date de péremption: 12 months

Détails sur eGFP

Antigène: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Autre désignation: EGFP

Sujet: EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Poids moléculaire: 27 kDa (for GFP without fusion partner)