VCAM1 anticorps (FITC)

N° du produit ABIN7835596

L’anticorps anti-VCAM1 Monoclonal Rat est utilisé pour la détection de VCAM1 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour VCAM1 anticorps (FITC) (ABIN7835596)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp VCAM1 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: M-K-2

Détail du produit anti-VCAM1 anticorps (FITC)

Fonction: Rat monoclonal antibody FITC labled to CD106

Specificité: Recognizes mouse CD106

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse preadipose cell line PA6

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur VCAM1

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM1

Sujet: L1CAM, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106

ID gène: 22329

Pathways: Carbohydrate Homeostasis