IL2 Receptor beta anticorps

N° du produit ABIN7835697

Cet anticorps Rat Monoclonal détecte spécifiquement IL2 Receptor beta dans WB, FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour IL2 Receptor beta anticorps (ABIN7835697)

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL2 Receptor beta est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TM-beta1

Détail du produit anti-IL2 Receptor beta anticorps

Fonction: Rat monoclonal antibody to CD122

Specificité: Recognizes mouse CD122

Purification: The antibody was purified by affinity chromatography.

Immunogène: Rat T cell line expressing mouse CD122 ( IL-2Rbeta)

Information d'application

Indications d'application: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur IL2 Receptor beta

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Autre désignation: IL2RB

Sujet: Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, p75, CD122

ID gène: 16185

Pathways: Signalistation JAK/STAT, Growth Factor Binding