IL7R anticorps

N° du produit ABIN7835711

Cet anticorps anti-IL7R Monoclonal Rat (Clone A7R34) (ABIN7835711) détecte spécifiquement IL7R dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour IL7R anticorps (ABIN7835711)

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL7R est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: A7R34

Détail du produit anti-IL7R anticorps

Fonction: Rat monoclonal antibody to CD127

Specificité: Recognizes mouse CD127

Purification: The antibody was purified by affinity chromatography.

Immunogène: mouse pre-B cell line 1A9

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur IL7R

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Autre désignation: IL7R

Sujet: Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD127

ID gène: 16197

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size