IL7R anticorps (Alexa Fluor 647)

N° du produit ABIN7835713

L’anticorps anti-IL7R Monoclonal Souris est utilisé pour la détection de IL7R dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour IL7R anticorps (Alexa Fluor 647) (ABIN7835713)

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL7R est conjugé à/à la Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: A019D5

Détail du produit anti-IL7R anticorps (Alexa Fluor 647)

Fonction: Mouse monoclonal antibody AF647 labled to CD127

Specificité: Recognizes human CD127

Purification: The antibody was purified by affinity chromatography.

Immunogène: Recombinant human CD127

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur IL7R

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Autre désignation: IL7R

Sujet: Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD127

ID gène: 3575

UniProt: P16871

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size