CD13 anticorps (Biotin)

N° du produit ABIN7835725

L’anticorps anti-CD13 Monoclonal Souris est utilisé pour la détection de CD13 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD13 anticorps (Biotin) (ABIN7835725)

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD13 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 46A11E3

Détail du produit anti-CD13 anticorps (Biotin)

Fonction: Mouse monoclonal antibody Biotin labled to CD13

Specificité: Recognizes human CD13

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD13.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD13

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Autre désignation: ANPEP

Sujet: APN, CD13, PEPN, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD antigen CD13

ID gène: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones