Syndecan 1 anticorps (APC)

N° du produit ABIN7835746

L’anticorps anti-Syndecan 1 Monoclonal Souris est utilisé pour la détection de Syndecan 1 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour Syndecan 1 anticorps (APC) (ABIN7835746)

Antigène: Syndecan 1 (SDC1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Syndecan 1 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: DL-101

Détail du produit anti-Syndecan 1 anticorps (APC)

Fonction: Mouse monoclonal antibody APC labled to CD138

Specificité: Recognizes human CD138

Purification: The antibody was purified by affinity chromatography.

Immunogène: A mixture of U266 and XG-1 human myeloma cell lines

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur Syndecan 1

Antigène: Syndecan 1 (SDC1)

Autre désignation: SDC1

Sujet: SDC, Syndecan-1, SYND1, CD138

ID gène: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism