CD200 anticorps

N° du produit ABIN7835935

L’anticorps anti-CD200 Monoclonal Rat est utilisé pour la détection de CD200 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour CD200 anticorps (ABIN7835935)

Antigène: CD200

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD200 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: OX-90

Détail du produit anti-CD200 anticorps

Fonction: Rat monoclonal antibody to CD200

Specificité: Recognizes mouse CD200

Purification: The antibody was purified by affinity chromatography.

Immunogène: Soluble fusion protein of the extracellular region of mouse OX-2 antigen with domains 3 and 4 of rat CD4 fusion protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD200

Antigène: CD200

Autre désignation: CD200

Sujet: MOX1, MOX2, OX-2 membrane glycoprotein, CD200

ID gène: 17470