CD22 anticorps (PE)

N° du produit ABIN7835966

Cet anticorps anti-CD22 Monoclonal Souris conjugué à PE (Clone BU59) (ABIN7835966) détecte spécifiquement CD22 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD22 anticorps (PE) (ABIN7835966)

Antigène: CD22 (CD22 Molecule (CD22))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD22 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BU59

Détail du produit anti-CD22 anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to CD22

Specificité: Recognizes human CD22

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD22.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD22

Antigène: CD22 (CD22 Molecule (CD22))

Autre désignation: CD22

Sujet: SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD antigen CD22

ID gène: 933

UniProt: P20273