PDCD1LG2 anticorps

N° du produit ABIN7836038

Cet anticorps anti-PDCD1LG2 Monoclonal Rat (Clone TY25) (ABIN7836038) détecte spécifiquement PDCD1LG2 dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour PDCD1LG2 anticorps (ABIN7836038)

Antigène: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp PDCD1LG2 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TY25

Détail du produit anti-PDCD1LG2 anticorps

Fonction: Rat monoclonal antibody to CD273

Specificité: Recognizes mouse CD273

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse B7-DC transfected cell line

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur PDCD1LG2

Antigène: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Autre désignation: PDCD1LG2

Sujet: B7DC, CD273, PDCD1L2, PDL2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC, CD273

ID gène: 58205