GP1BB anticorps (APC)

N° du produit ABIN7836301

L’anticorps anti-GP1BB Monoclonal Souris est utilisé pour la détection de GP1BB dans des échantillons de Humain. Il a été validé pour IF et FACS.

Aperçu rapide pour GP1BB anticorps (APC) (ABIN7836301)

Antigène: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GP1BB est conjugé à/à la APC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HIP1

Détail du produit anti-GP1BB anticorps (APC)

Fonction: Mouse monoclonal antibody APC labled to CD42b

Specificité: Recognizes human CD42b

Purification: The antibody was purified by affinity chromatography.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur GP1BB

Antigène: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Autre désignation: GP1BB

Sujet: Platelet glycoprotein Ib beta chain, GP-Ib beta, GPIb-beta, GPIbB, Antigen CD42b-beta, CD42c

ID gène: 2812

UniProt: P13224