ITGA4 anticorps

N° du produit ABIN7836413

Cet anticorps anti-ITGA4 Monoclonal Rat (Clone PS-2) (ABIN7836413) détecte spécifiquement ITGA4 dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour ITGA4 anticorps (ABIN7836413)

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA4 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PS-2

Détail du produit anti-ITGA4 anticorps

Fonction: Rat monoclonal antibody to CD49d

Specificité: Recognizes mouse CD49d

Purification: The antibody was purified by affinity chromatography.

Immunogène: AKR/Cum mouse spontaneous T lymphoma line TK1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur ITGA4

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Autre désignation: ITGA4

Sujet: CD49D, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, CD49d

ID gène: 16401

Pathways: Integrin Complex