ITGA4 anticorps (APC)

N° du produit ABIN7836415

L’anticorps anti-ITGA4 Monoclonal Souris est utilisé pour la détection de ITGA4 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour ITGA4 anticorps (APC) (ABIN7836415)

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA4 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BU49

Détail du produit anti-ITGA4 anticorps (APC)

Fonction: Mouse monoclonal antibody APC labled to CD49d

Specificité: Recognizes human CD49d

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD49d.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur ITGA4

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Autre désignation: ITGA4

Sujet: CD49D, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, CD antigen CD49d

ID gène: 3676

UniProt: P13612

Pathways: Integrin Complex