CD55 anticorps (PE-Cy5)

N° du produit ABIN7836469

L’anticorps anti-CD55 Monoclonal Souris est utilisé pour la détection de CD55 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD55 anticorps (PE-Cy5) (ABIN7836469)

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD55 est conjugé à/à la PE-Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 143-30

Détail du produit anti-CD55 anticorps (PE-Cy5)

Fonction: Mouse monoclonal antibody PE/Cy5 labled to CD55

Specificité: Recognizes human CD55

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD55.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD55

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Autre désignation: CD55

Sujet: CR, DAF, Complement decay-accelerating factor, CD antigen CD55

ID gène: 1604

UniProt: P08174

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity