CD70 anticorps

N° du produit ABIN7836565

L’anticorps anti-CD70 Monoclonal Rat est utilisé pour la détection de CD70 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour CD70 anticorps (ABIN7836565)

Antigène: CD70 (CD70 Molecule (CD70))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD70 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: FR70

Détail du produit anti-CD70 anticorps

Fonction: Rat monoclonal antibody to CD70

Specificité: Recognizes mouse CD70

Purification: The antibody was purified by affinity chromatography.

Immunogène: BALB/c mouse B lymphoma A20.2J

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD70

Antigène: CD70 (CD70 Molecule (CD70))

Autre désignation: CD70

Sujet: CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70

ID gène: 21948

Pathways: Cancer Immune Checkpoints