CD8 alpha anticorps (PerCP-Cy5.5)

N° du produit ABIN7836612

Cet anticorps Souris Monoclonal détecte spécifiquement CD8 alpha dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD8 alpha anticorps (PerCP-Cy5.5) (ABIN7836612)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la PerCP-Cy5.5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SK1

Détail du produit anti-CD8 alpha anticorps (PerCP-Cy5.5)

Fonction: Mouse monoclonal antibody PerCP/Cy5.5 labled to CD8

Specificité: Recognizes human CD8

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD8.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8A

Sujet: MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD antigen CD8b

ID gène: 925

UniProt: P01732

Pathways: TCR Signaling