HLA-B27 anticorps (PE)

N° du produit ABIN7839659

Cet anticorps anti-HLA-B27 Monoclonal Souris conjugué à PE (Clone HLA-B27) (ABIN7839659) détecte spécifiquement HLA-B27 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour HLA-B27 anticorps (PE) (ABIN7839659)

Antigène: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-B27 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HLA-B27

Détail du produit anti-HLA-B27 anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to HLA-B27

Specificité: Recognizes human HLA-B27

Purification: The antibody was purified by affinity chromatography.

Immunogène: Immune complex precipitated from HLA-B27 positive cell line (Bordin)

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur HLA-B27

Antigène: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Autre désignation: HLA-B27

Sujet: HLA-B27 protein

UniProt: O19193

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response