IL1A anticorps

N° du produit ABIN7840001

Cet anticorps anti-IL1A Monoclonal Hamster arménien (Clone ALF-161) (ABIN7840001) détecte spécifiquement IL1A dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour IL1A anticorps (ABIN7840001)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp IL1A est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ALF-161

Détail du produit anti-IL1A anticorps

Fonction: Armenian Hamster monoclonal antibody to IL-1 alpha

Specificité: Recognizes mouse IL-1 alpha

Purification: The antibody was purified by affinity chromatography.

Immunogène: E. coli - expressed, recombinant mouse IL-1

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur IL1A

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL1A

Sujet: IL1F1, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

ID gène: 16175

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints