IL12B anticorps

N° du produit ABIN7840010

Cet anticorps anti-IL12B Monoclonal Souris (Clone ABT-874) (ABIN7840010) détecte spécifiquement IL12B dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour IL12B anticorps (ABIN7840010)

Antigène: IL12B (Interleukin 12 beta (IL12B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL12B est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ABT-874

Détail du produit anti-IL12B anticorps

Fonction: Mouse monoclonal antibody to IL-12 p40

Specificité: Recognizes human IL-12 p40

Purification: The antibody was purified by affinity chromatography.

Immunogène: Recombinant human IL-12 (p40)

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur IL12B

Antigène: IL12B (Interleukin 12 beta (IL12B))

Autre désignation: IL12B

Sujet: NKSF2, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2

ID gène: 3593

UniProt: P29460

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation