IL-5 anticorps

N° du produit ABIN7840044

Cet anticorps Rat Monoclonal détecte spécifiquement IL-5 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour IL-5 anticorps (ABIN7840044)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-5 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TRFK5

Détail du produit anti-IL-5 anticorps

Fonction: Rat monoclonal antibody to IL-5

Specificité: Recognizes human/mouse IL-5

Purification: The antibody was purified by affinity chromatography.

Immunogène: Partially-purified T cell clone supernatant

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL5

Sujet: Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF

ID gène: 3567, 16191

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour