Integrin beta 7 anticorps

N° du produit ABIN7840105

L’anticorps anti-Integrin beta 7 Monoclonal Rat est utilisé pour la détection de Integrin beta 7 dans des échantillons de Humain et Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour Integrin beta 7 anticorps (ABIN7840105)

Antigène: Integrin beta 7 (ITGB7)

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 7 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: FIB21

Détail du produit anti-Integrin beta 7 anticorps

Fonction: Rat monoclonal antibody to Integrin beta 7

Specificité: Recognizes human/mouse Integrin beta 7

Purification: The antibody was purified by affinity chromatography.

Immunogène: TK1 cells

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur Integrin beta 7

Antigène: Integrin beta 7 (ITGB7)

Autre désignation: ITGB7

Sujet: Integrin beta-7, Gut homing receptor beta subunit

ID gène: 3695, 16421

UniProt: P26010

Pathways: Integrin Complex